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p65-expressing dna constructs rela-flag  (Addgene inc)


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    Addgene inc p65-expressing dna constructs rela-flag
    P65 Expressing Dna Constructs Rela Flag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p65-expressing dna constructs rela-flag/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    p65-expressing dna constructs rela-flag - by Bioz Stars, 2026-06
    90/100 stars

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    p65-expressing dna constructs rela-flag  (Addgene inc)
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    Addgene inc p65-expressing dna constructs rela-flag
    P65 Expressing Dna Constructs Rela Flag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
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    (A) Serum LPS, (B) liver Tlr4 mRNA, and (C) liver <t>p65</t> protein expression in WT mice fed chow or NASH diet for 16 weeks (n = 3 to 6 per group). (D) Jag1 promoter luciferase activity in WT primary hepatocytes cotransfected with p65 (n = 4 per group), and (E) ChIP assay for NF-κB occupancy at the Jag1 promoter in livers from mice fed normal chow or NASH diet for 16 weeks (n = 4 per group). (F) Eight- to 10-week-old Tlr4flox/flox mice were transduced with AAV8-Tbg-GFP (Cre−) or AAV8-Tbg-CRE (L-Tlr4) and then fed NASH diet for 16 weeks (n = 12 to 13 per group). (G) Jagged1 protein expression, (H) expression of Notch targets, and (I) markers of HSC activation. (J) Liver Sirius red staining (n = 4 to 9 per group). (K) Control (pLive-con) or Jag1 expression (pLive-Jagged1) vectors were hydrodynamically administered to adult Tlr4flox/flox mice. Two weeks later, all mice were transduced with AAV8-Tbg-CRE and then fed NASH diet for 16 weeks (n = 8 per group). (L) Expression of Jag1 mRNA and (M) markers of HSC activity. (N) Sirius red staining (n = 5 per group). *P < 0.05, **P < 0.01, and ***P < 0.001 as compared to the indicated controls by two-tailed t tests (two groups). All data are presented as means ± SEM.
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    ms2 p65 hsf1 activator expression construct  (Addgene inc)
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    (A) Serum LPS, (B) liver Tlr4 mRNA, and (C) liver <t>p65</t> protein expression in WT mice fed chow or NASH diet for 16 weeks (n = 3 to 6 per group). (D) Jag1 promoter luciferase activity in WT primary hepatocytes cotransfected with p65 (n = 4 per group), and (E) ChIP assay for NF-κB occupancy at the Jag1 promoter in livers from mice fed normal chow or NASH diet for 16 weeks (n = 4 per group). (F) Eight- to 10-week-old Tlr4flox/flox mice were transduced with AAV8-Tbg-GFP (Cre−) or AAV8-Tbg-CRE (L-Tlr4) and then fed NASH diet for 16 weeks (n = 12 to 13 per group). (G) Jagged1 protein expression, (H) expression of Notch targets, and (I) markers of HSC activation. (J) Liver Sirius red staining (n = 4 to 9 per group). (K) Control (pLive-con) or Jag1 expression (pLive-Jagged1) vectors were hydrodynamically administered to adult Tlr4flox/flox mice. Two weeks later, all mice were transduced with AAV8-Tbg-CRE and then fed NASH diet for 16 weeks (n = 8 per group). (L) Expression of Jag1 mRNA and (M) markers of HSC activity. (N) Sirius red staining (n = 5 per group). *P < 0.05, **P < 0.01, and ***P < 0.001 as compared to the indicated controls by two-tailed t tests (two groups). All data are presented as means ± SEM.
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    cdna construct expressing full-length p65 n-terminally tagged with egfp  (Addgene inc)
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    (A) Serum LPS, (B) liver Tlr4 mRNA, and (C) liver <t>p65</t> protein expression in WT mice fed chow or NASH diet for 16 weeks (n = 3 to 6 per group). (D) Jag1 promoter luciferase activity in WT primary hepatocytes cotransfected with p65 (n = 4 per group), and (E) ChIP assay for NF-κB occupancy at the Jag1 promoter in livers from mice fed normal chow or NASH diet for 16 weeks (n = 4 per group). (F) Eight- to 10-week-old Tlr4flox/flox mice were transduced with AAV8-Tbg-GFP (Cre−) or AAV8-Tbg-CRE (L-Tlr4) and then fed NASH diet for 16 weeks (n = 12 to 13 per group). (G) Jagged1 protein expression, (H) expression of Notch targets, and (I) markers of HSC activation. (J) Liver Sirius red staining (n = 4 to 9 per group). (K) Control (pLive-con) or Jag1 expression (pLive-Jagged1) vectors were hydrodynamically administered to adult Tlr4flox/flox mice. Two weeks later, all mice were transduced with AAV8-Tbg-CRE and then fed NASH diet for 16 weeks (n = 8 per group). (L) Expression of Jag1 mRNA and (M) markers of HSC activity. (N) Sirius red staining (n = 5 per group). *P < 0.05, **P < 0.01, and ***P < 0.001 as compared to the indicated controls by two-tailed t tests (two groups). All data are presented as means ± SEM.
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    Average 90 stars, based on 1 article reviews
    cdna construct expressing full-length p65 n-terminally tagged with egfp - by Bioz Stars, 2026-06
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    cdna construct expressing full-length p65 n-terminally tagged egfp  (Addgene inc)
    90
    Addgene inc cdna construct expressing full-length p65 n-terminally tagged egfp
    (A) Serum LPS, (B) liver Tlr4 mRNA, and (C) liver <t>p65</t> protein expression in WT mice fed chow or NASH diet for 16 weeks (n = 3 to 6 per group). (D) Jag1 promoter luciferase activity in WT primary hepatocytes cotransfected with p65 (n = 4 per group), and (E) ChIP assay for NF-κB occupancy at the Jag1 promoter in livers from mice fed normal chow or NASH diet for 16 weeks (n = 4 per group). (F) Eight- to 10-week-old Tlr4flox/flox mice were transduced with AAV8-Tbg-GFP (Cre−) or AAV8-Tbg-CRE (L-Tlr4) and then fed NASH diet for 16 weeks (n = 12 to 13 per group). (G) Jagged1 protein expression, (H) expression of Notch targets, and (I) markers of HSC activation. (J) Liver Sirius red staining (n = 4 to 9 per group). (K) Control (pLive-con) or Jag1 expression (pLive-Jagged1) vectors were hydrodynamically administered to adult Tlr4flox/flox mice. Two weeks later, all mice were transduced with AAV8-Tbg-CRE and then fed NASH diet for 16 weeks (n = 8 per group). (L) Expression of Jag1 mRNA and (M) markers of HSC activity. (N) Sirius red staining (n = 5 per group). *P < 0.05, **P < 0.01, and ***P < 0.001 as compared to the indicated controls by two-tailed t tests (two groups). All data are presented as means ± SEM.
    Cdna Construct Expressing Full Length P65 N Terminally Tagged Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna construct expressing full-length p65 n-terminally tagged egfp/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    cdna construct expressing full-length p65 n-terminally tagged egfp - by Bioz Stars, 2026-06
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    (A) Serum LPS, (B) liver Tlr4 mRNA, and (C) liver p65 protein expression in WT mice fed chow or NASH diet for 16 weeks (n = 3 to 6 per group). (D) Jag1 promoter luciferase activity in WT primary hepatocytes cotransfected with p65 (n = 4 per group), and (E) ChIP assay for NF-κB occupancy at the Jag1 promoter in livers from mice fed normal chow or NASH diet for 16 weeks (n = 4 per group). (F) Eight- to 10-week-old Tlr4flox/flox mice were transduced with AAV8-Tbg-GFP (Cre−) or AAV8-Tbg-CRE (L-Tlr4) and then fed NASH diet for 16 weeks (n = 12 to 13 per group). (G) Jagged1 protein expression, (H) expression of Notch targets, and (I) markers of HSC activation. (J) Liver Sirius red staining (n = 4 to 9 per group). (K) Control (pLive-con) or Jag1 expression (pLive-Jagged1) vectors were hydrodynamically administered to adult Tlr4flox/flox mice. Two weeks later, all mice were transduced with AAV8-Tbg-CRE and then fed NASH diet for 16 weeks (n = 8 per group). (L) Expression of Jag1 mRNA and (M) markers of HSC activity. (N) Sirius red staining (n = 5 per group). *P < 0.05, **P < 0.01, and ***P < 0.001 as compared to the indicated controls by two-tailed t tests (two groups). All data are presented as means ± SEM.

    Journal: Science translational medicine

    Article Title: Hepatocyte TLR4 triggers inter-hepatocyte Jagged1/Notch signaling to determine NASH-induced fibrosis

    doi: 10.1126/scitranslmed.abe1692

    Figure Lengend Snippet: (A) Serum LPS, (B) liver Tlr4 mRNA, and (C) liver p65 protein expression in WT mice fed chow or NASH diet for 16 weeks (n = 3 to 6 per group). (D) Jag1 promoter luciferase activity in WT primary hepatocytes cotransfected with p65 (n = 4 per group), and (E) ChIP assay for NF-κB occupancy at the Jag1 promoter in livers from mice fed normal chow or NASH diet for 16 weeks (n = 4 per group). (F) Eight- to 10-week-old Tlr4flox/flox mice were transduced with AAV8-Tbg-GFP (Cre−) or AAV8-Tbg-CRE (L-Tlr4) and then fed NASH diet for 16 weeks (n = 12 to 13 per group). (G) Jagged1 protein expression, (H) expression of Notch targets, and (I) markers of HSC activation. (J) Liver Sirius red staining (n = 4 to 9 per group). (K) Control (pLive-con) or Jag1 expression (pLive-Jagged1) vectors were hydrodynamically administered to adult Tlr4flox/flox mice. Two weeks later, all mice were transduced with AAV8-Tbg-CRE and then fed NASH diet for 16 weeks (n = 8 per group). (L) Expression of Jag1 mRNA and (M) markers of HSC activity. (N) Sirius red staining (n = 5 per group). *P < 0.05, **P < 0.01, and ***P < 0.001 as compared to the indicated controls by two-tailed t tests (two groups). All data are presented as means ± SEM.

    Article Snippet: The p65 expression construct ( 72 ) was obtained from Addgene (no. 23255).

    Techniques: Expressing, Luciferase, Activity Assay, Transduction, Activation Assay, Staining, Control, Two Tailed Test